Contribution
Acoustofluidics for 3D cell cultures
* Presenting author
Abstract:
Microscale acoustofluidics at MHz frequencies can be used for cell manipulation in microfluidic devices. We use this technology in multi-well microplates for the purpose of generating 3D cell cultures, or spheroids, with the purpose of using the cultures as tumor models in cancer therapy research. The method utilizes acoustic radiation forces from standing-wave ultrasonic fields in hundreds of sub-mm-scaled wells etched in glass-silicon chips. The chips are designed for live cell microscopy, and the developing 3D cell cultures can be continuously imaged during growth and maturation. The method is used for studying the interaction at high temporal and spatial resolution between the micro-tumors (spheroids) and anti-cancer drugs and immune cells.